JOURNAL PAPER

Md Alamgir Kabir, Ashish Kharel, Saloni Malla,Zachary Joseph Kreis, Peuli Nath, Jared Neil Wolfe, Marwa Hassan, Devinder Kaur, Hamed Sari-Sarraf, Amit K. Tiwari, Aniruddha Ray, Automated detection of apoptotic versus non-apoptotic cell death using label-free computational microscopy, Journal of Biophotonics, doi: https://doi.org/10.1002/jbio.202100310

Identification of cell death mechanisms, particularly distinguishing between apoptotic versus non-apoptotic pathways, is of paramount importance for a wide range of applications related to cell signaling, interaction with pathogens, therapeutic processes, drug discovery, drug resistance and even pathogenesis of diseases like cancers and neurogenerative disease among others. Here we present a novel high-throughput method of identifying apoptotic vs necrotic vs other non-apoptotic cell death processes, based on lensless digital holography. This method relies on identification of the temporal changes in the morphological features of mammalian cells, which are unique to each cell death processes. Different cell death processes were induced by known cytotoxic agents. A deep learning-based approach was used to automatically classify the cell death mechanism (apoptotic vs necrotic vs non-apoptotic) with more than 93% accuracy. This label free approach can provide a low cost (<$250) alternative to some of the currently available high content imaging-based screening tools.

Peuli Nath, Md Alamgir Kabir, Somaiyeh Khoubafarin Doust, Aniruddha Ray, Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques, Infectious Disease Reports, 13, 2, pp.518-539, 2021. doi: https://doi.org/10.3390/idr13020049

Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

Khadiza Begam, Mohammad Moshiur Rahman, Mohammad Alamgir Kabir, Umma Tamim, Syed Mohammod Hossain, Afia Begum, Natural Radioactivity Level of 238U, 232Th, and 40K in Baby Food and Committed Annual Effective Dose Assessment in Bangladesh, International Journal of Environmental Monitoring and Analysis, 8, 6, pp.187-192, 2020.

Worldwide, the contagion of chronic diseases like diabetes and cancer among children has made the nutritionists thoughtful. Food contamination with radioactivity, became a severe health threat for children below five since they are under developing immune systems and cannot fight off infections like adults. Radioactivity concentrated in food chain may be transferred to human body and increase the cumulative risk of developing cancer and some other diseases. Therefore the assessment of radioactivity levels in baby food and their associated doses are of crucial importance for health safety. The study is focused on the radiation contamination of baby food due to the nuclear disasters and make public awareness about infant’s nutrition followed by the health safety. The natural radioactivity level due to 238U, 232Th and 40K in baby food (cereals and powder milk) samples, marketed in Bangladesh were estimated and annual effective dose was assessed. Gamma spectrometry was performed by HPGe detector coupled with MCA, and certified reference materials were used for quantification and quality control. The average concentrations of 238U, 232Th and 40K were found to be 5.42±0.42, 5.71±0.50 and 334.4±12 Bq. Kg-1 in milk sample and 2.98±0.38, 3.94±0.29 and 155.7±7.5 Bq. Kg-1 in cereal sample respectively. The committed effective doses due to ingestion of natural radionuclides (238U, 232Th and 40K) from the consumption of milk and cereals for age group< 1 year were 1.30 and 0.51 mSv/yr and for age group 1-2 year were 0.31 and 0.15 mSv/yr respectively. The age group< 1yr, who are completely depend on formula milk are under …

Peuli Nath, Alamgir Kabir, Somaiyeh Khoubafarin Doust, Zachary Joseph Kreais, Aniruddha Ray, Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices, Diagnostics, 10, 10, pp.871, 2020. doi: https://doi.org/10.3390/diagnostics10100841

Infectious diseases caused by bacteria and viruses are highly contagious and can easily be transmitted via air, water, body fluids, etc. Throughout human civilization, there have been several pandemic outbreaks, such as the Plague, Spanish Flu, Swine-Flu, and, recently, COVID-19, amongst many others. Early diagnosis not only increases the chance of quick recovery but also helps prevent the spread of infections. Conventional diagnostic techniques can provide reliable results but have several drawbacks, including costly devices, lengthy wait time, and requirement of trained professionals to operate the devices, making them inaccessible in low-resource settings. Thus, a significant effort has been directed towards point-of-care (POC) devices that enable rapid diagnosis of bacterial and viral infections. A majority of the POC devices are based on plasmonics and/or microfluidics-based platforms integrated with mobile readers and imaging systems. These techniques have been shown to provide rapid, sensitive detection of pathogens. The advantages of POC devices include low-cost, rapid results, and portability, which enables on-site testing anywhere across the globe. Here we aim to review the recent advances in novel POC technologies in detecting bacteria and viruses that led to a breakthrough in the modern healthcare industry

Characterization of Electro Active Polymer Composite Films Based on Gelatin and Poly 2-(3-thienyl)-ethoxy-4-butylsulfonate (PTEBS),

Condensed Matter

Properties of Proton Exchange Membranes Poly-ethylene Terephthalate (PET) Films Developed by Gamma Radiation Induced Grafting and Sulfonation Technique,

Polymer Science

Preparation of Unidirectional Jute Fiber Reinforced PVC Film Composites and Study of Their Different Properties,

Polymer Physics

Optical, Electrical and Thermal Properties of Jute and Glass Fiber Reinforced LDPE Composites,

Polymer Physics

Comparative Study of Physical And Elastic Properties of Jute And Glass Fiber Reinforced LDPE Composites,

Polymer Physics

Elastic and Electrical Properties of Graphite and Talc Filler Reinforced Polypropylene (PP) Composites,

Polymer Physics

Physical, Optical and Thermal Properties of Graphite and Talc Filler Reinforced Polypropylene (PP) Composites,

Polymer Physics

Optical and Morphological Characterization of BaSeThin Films Synthesized via Chemical Bath Deposition,

Materials Science

Study on Physio-Mechanical Properties of Rice Husk Ash Polyester Resin Composite,

Polymer Physics

Optical, Structural and Morphological Properties of Spin Coated Copper Zinc Tin Sulfide Thin Films,

Condensed Matter Physics

Assessment of Natural Radioactivity Levels and Radiological Significance of Bottled Drinking Water in Bangladesh,

Nuclear Physics

Influence of calcination on the sol–gel synthesis of lanthanum oxide nanoparticle,

Nanomaterials

Polyethylene glycol assisted facile sol-gel synthesis of lanthanum oxide nanoparticles: Structural characterizations and photoluminescence studies,

Nanomaterials 

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