Mohammad Mahfuz Ali Khan Shawan Assistant Professor, Department of Biochemistry & Molecular Biology
PROFILE
SHORT BIOGRAPHY
Mohammad Mahfuz Ali Khan Shawan is an Assistant Professor in the Department of Biochemistry and Molecular Biology at Jahangirnagar University, Savar, Dhaka, Bangladesh. He completed his SSC in 2003 from Govt. Science College Attached High School and his HSC in 2005 from Notre Dame College. He earned his B.Sc. and M.Sc. degrees in Biochemistry and Molecular Biology from Jahangirnagar University in 2011 and 2012, respectively. For his M.Sc. thesis, Mr. Shawan conducted research in the Environmental Microbiology Laboratory at the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). He then worked as a Research Officer at icddr,b until October 2014, focusing on antimicrobial resistance in Vibrio cholerae.
In late 2014, Mr. Shawan joined Jahangirnagar University as a Lecturer in the Department of Biochemistry and Molecular Biology. He teaches both theoretical and practical classes to students from the first year to the MS level. He also supervises several research/thesis students working on topics such as bioinformatics, microbiology, biomedical engineering, biochemistry, and molecular biology. His research interests include Biochemistry, Molecular Biology, Microbiology, Cell Biology, Genetic Engineering, Biotechnology, Immunology, and Bioinformatics. Mr. Shawan has published over 31 scientific articles and a book chapter in well-reputed peer-reviewed journals (American Chemical Society, Sprinegr, Elsevier, Bentham Science, Hindawe, Bioinformation et.) and serves as a reviewer for several renowned journals (Scientific Reports, PlosOne, Archives of Microbiology, Biotecnology Letters etc.). He has participated in around eight conferences and workshops.
Born on July 20, 1987, in Bangladesh, Mr. Shawan currently resides with his family in Dhaka city. He enjoys traveling, reading, singing, internet surfing, watching movies, sports, and visiting exhibitions.
RESEARCH INTEREST
Biochemistry, Molecular Biology, Microbiology, Cell Biology, Genetic Engineering, Biotechnology, Neurobiology, Cancer Biology, Immunology, and Bioinformatics.
All Publications
2024 |
1. Halder, SK; Sharma, AR; Arian, TA; Saha, S; Shil, A; Rafi, MO; Sarker, SR; Alam, MN; Himel, MK; Hasan, MA and Shawan, MMAK*. In silico Designing of a Multi-epitope-based Subunit Vaccine against SARS-CoV-2 (Delta Variant) by Exploiting Its Structural Proteins: A Reverse Vaccinomics and Immunoinformatics Approach. Coronaviruses. 2024; 5(4). |
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2023 |
2. Shawan, MMAK*; Sharma, AR; Halder, SK; Arian, TA; Shuvo, MN; Sarker, SR and Hasan, MA. Advances in Computational and Bioinformatics Tools and Databases for Designing and Developing a Multi‑Epitope‑Based Peptide Vaccine. International Journal of Peptide Research and Therapeutics. 2023; 29(60): 1-20. 3. Halder, SK; Sultana, I; Shuvo, MZ; Shil, A; Himel, MK; Hasan, MA and Shawan, MMAK*. In Silico Identification and Analysis of Potentially Bioactive Antiviral Phytochemicals against SARS-CoV-2: A Molecular Docking and Dynamics Simulation Approach. BioMed Res. Int. 2023; 2023: 1-32. 4. Shakil, MS; Uddin, MF; Morshed, MR; Bhuiya, MS; Alam, M; Hossen, MS; Niloy, MS; Shawan, MMAK; Hoque, SM and Hasan, MA. Progeny Transfer Effects of Chitosan-Coated Cobalt Ferrite Nanoparticles. ACS Omega. 2023; 8(17): 15152-15159. 5. Ayon, RA; Faike, MA; Ononna, SZ; Hassan, A; Kundu, S; Akhter, F; Shawan, MMAK; Hossain, MM; Khalil, MI and Hasan, MA. An overview of the risk, underlying factors, and mechanism of cancer progression in polycystic ovary syndrome. Tumor Discovery. 2023; 2(1): 328. |
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2022 |
6. Mahmud, KM; Hossain, MM; Polash, SA; Takikawa, M; Shakil, MS; Uddin, MF; Alam, M; Shawan, MMAK; Saha, T; Takeoka, S; Hasan, MA and Sarker, SR. Investigation of Antimicrobial Activity and Biocompatibility of Biogenic Silver Nanoparticles Synthesized using Syzigyum cymosum Extract. ACS Omega. 2022; 7(31): 27216-27229. 7. Jahan, N; Ahamed, T; Das, A; Khan, MA; Hossain, S; Sarker, SR and Shawan, MMAK*. In Silico Subtractive Genomics Approach Characterizes a Hypothetical Protein (MG_476) from Mycoplasma genitalium G37. Journal of Clinical and Experimental Investigations. 2022; 13(4): 1-10. |
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2021 |
8. Hossain, M; Hasan, A, Shawan, MMAK; Banik, S and Jahan, I. Current Therapeutic Strategies of Xeroderma Pigmentosum. Indian J. Dermatol. 2021; 66: 660-7 9. Shawan, MMAK; Sharma, AR; Bhattacharya, M; Mallik, B; Akhter, F; Shakil, MS; Hossain, MM; Banik, S; Lee, SS; Hasan, MA and Chakraborty, C. Designing an effective therapeutic siRNA to silence RdRp gene of SARS-CoV-2. Infection, Genetics and Evolution. 2021; 93(104951): 1-14. |
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10. Parvin, A; Debnath, P; Hasan, MM; Rokunozzaman; Hasan, MA; Shawan, MMAK; Hossain, MM; Rahman, MN; Khalil, MI and Ahmed, S. Antibiotic resistance in heavy metal resistant nonlactose fermenting Enterobacteriaceae colonies cultured from textile industry effluents. Microbiology Research International. 2021; 9(2): 21-39. 11. Rokunozzaman; Parvin, A; Hasan, MM; Debnath, P; Hasan, MA; Hossain, MM; Shawan, MMAK; Halim, A; Rahman, MA; Hossain, S and Ahmed, S. Role of heavy metals on antibiotic resistance properties in lactose fermenting isolates collected from textile industry effluents. African Journal of Microbiology Research. 2021; 15(5): 236-252. |
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12. Shawan, MMAK*; Halder, SK and Hasan, MA. Luteolin and abyssinone II as potential inhibitors of SARS-CoV-2: an in silico molecular modeling approach in battling the COVID-19 outbreak. Bull. Natl. Res. Cent., 2021; 45(27): 1-21. |
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2020 |
13. Niloy, MS; Hossain, MM; Takikawa, M; Shakil, MS; Polash, SA; Mahmud, KM; Uddin, MF; Alam, M; Shubhra, RD; Shawan, MMAK; Saha, T; Takeoka, S; Hasan, MA and Sarker, SR. Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility. ACS Appl. Bio Matter., 2020; 3(11): 7722-7733. |
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2019
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14. Ahmed, SJ; Hasan, MA; Islam, MR; Shawan, MMAK; Uddin, MF; Rahman, MN and Hossain, MM. Prevalence and antibiotic susceptibility profile of Pasteurella maltocida isolates isolated from goats in Savar area of Bangladesh. Agricultural Science Digest, 2019; 39(4): 357-360. |
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15. Ashrafi, H; Siraji, MI; Showva, NN; Hossain, MM; Hossan, T; Hasan, MA; Shohael, AM and Shawan, MMAK*. Structure to function analysis with antigenic characterization of a hypothetical protein, HPAG1_0576 from Helicobacter pylori HPAG1. Bioinformation, 2019; 15(7): 456-466. 16. Shawan, MMAK; Islam, N; Aziz, S; Khatun, N; Sarker, SR; Hossain, M; Hossan, T; Morshed, M; Sarkar, M; Shakil, MS; Rahman, MN; Begum, MHA and Hasan, MA. Fabrication of Xanthan gum:Gelatin (Xnt:Gel) Hybrid Composite Hydrogels for Evaluating Skin Wound Healing Efficacy. Modern Applied Science, 2019; 13(3): 101-111. |
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2018 |
17. Saha, M; Ahammad, H; Bhoumik, NC; Shakil, MS; Shawan, MMAK; Morshed, M; Hossan, T; Sarker, SR; Rahman, MN; Rahman, SMB; Hossain, MM and Hasan, MA. Extraction and estimation of alpha lipoic acid content in different food samples by reverse phase HPLC: effect of heat treatment. International Journal of Biosciences, 2018; 13(5): 473-482. 18. Tasnim, M; Ahammed, R; Shawan, MMAK; Hasan, A; Islam, MS and Morshed, M. A comparative study on lipid profile of before and after synchronized dairy cows under bathan rearing system at Sirajgonj district of Bangladesh. International Journal of Biosciences, 2018; 13(5): 301-308. 19. Shawan, MMAK*; Hasan, MA; Yesmin, R; Hossan, T; Hossain, MM; Hasan, MM; Parvin, A; Morshed, M; Salauddin, NM; Sarker, SR; Rahman, MN and Rahman, SMB. tRNA diversification among uncultured archeon clones. Bioinformation, 2018; 14(7): 357-360. |
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2016 |
20. Hossain, MM; Jahan, I; Akter, S; Hasan, MM; Uddin, KR; Shawan, MMAK; Parvin, A; Hasan, MA; Ibrahim, M and Helali, MOH. Ethanol content in different energy drinks available in Bangladesh. Jahangirnagar University J. Biol. Sci., 2016; 5(2): 57-60. 21. Shawan, MMAK*; Hasan, MA; Hossain, MM; Hasan, MM; Parvin, A; Akter, S; Uddin, KR; Banik, S; Morshed, M; Rahman, MN and Rahman, SMB. Genomics Dataset on Unclassified Published Organism (patent US 7547531). Data in Brief, 2016; 9: 602-605. 22. Rahman, A; Ali, MT; Shawan, MMAK; Sarwar, MG; Khan, MAK and Halim, MA. Halogen-directed drug design for Alzheimer’s disease: a combined density functional and molecular docking study. SpringerPlus, 2016; 5: 1346. 23. Islam, MM; Hasan, MA; Yousuf, MA; Islam, UK; Shawan, MMAK and Islam, MR. Seroprevalence of Peste des Petits Ruminant Virus specific antibody in goats in different regions of Bangladesh. J. Adv. Vet. Anim. Res., 2016; 3(2): 127-133. 24. Disha, JS; Begum, MHA; Shawan, MMAK; Khatun, N; Ahmed, S; Islam, MS; Karim, MR; Islam, MR; Hossain, MM and Hasan, MA. Preparation and Characterization of Xanthan Gum-based Biodegradable Polysaccharide Hydrogels. Res. J. Material Sci., 2016; 4(4): 13-18. 25. Shawan, MMAK*; Mahmud, HA; Gope, PS; Salauddin, NM; Rahman, MH; Ahmed, MA; Nafiz, TN; Imran, KM; Rahman, MN and Rahman, SMB. Campylobacter jejuni ATCC 700819: An in silico Approach to Identify and Categorize Probable Drug Targets by Subtractive Genome Analysis. J. PURE APPL. MICROBIO., 2016; 10(1): 241-241. |
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2015 |
26. Shawan, MMAK*; Hossain, MM; Hasan, MM; Parvin, A; Akter, S; Uddin, KR; Banik, S; Morshed, M; Hasan, MA; Rahman, MN and Rahman, SMB. In silico Characterization and Investigation of Putative Promoter Motifs in Ebolavirus Genome. Journal of Global Biosciences, 2015; 4(3): 1747-1757. 27. Hossain, MM; Jahan, I; Shawan, MMAK; Parvin, A; Hasan, MM; Uddin, KR; Akter, S; Banik, S; Hasan, MA; Morshed, M; Rahman, MN and Rahman, SMB. Determination of pH, caffeine and reducing sugar in energy drinks available in Bangladesh. N. Y. Sci. J., 2015; 8(2): 92-96. 28. Shawan, MMAK*; Hossain, MM; Hasan, MA; Hasan, MM; Parvin, A; Akter, S; Uddin, KR; Banik, S; Morshed, M; Rahman, MN and Rahman, SMB. Design and Prediction of Potential RNAi (siRNA) Molecules for 3'UTR PTGS of Different Strains of Zika Virus: A Computational Approach. Nat. Sci., 2015; 13(2): 37-50. |
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2014 |
29. Shawan, MMAK*; Mahmud, HA; Hasan, MM; Parvin, A; Rahman, MN and Rahman, SMB. In Silico Modeling and Immunoinformatics Probing Disclose the Epitope Based Peptide Vaccine Against Zika Virus Envelope Glycoprotein. Indian Journal of Pharmaceutical and Biological Research (IJPBR), 2014; 2(4): 44-57. |
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2013 |
30. Aziz, MA; Shawan, MMAK; Rahman, S; Islam, T; Mita, M; Faruque, A; and Rana, MS. Secondary metabolites, antimicrobial, brine shrimp lethality & 4th instar Culex quinquefasciatus mosquito larvicidal screening of organic & inorganic root extracts of Microcos paniculata. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 2013; 8(5): 58-65. |
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2009 |
31. Rahman, A; Hossan, T; Amin, SMR; Rahman, KA; Shawan, MMAK and Khalil, I. Antimicrobial Activity of Pleurotus ostreatus (Jacquin ex Fr.) Kummer upon Human Pathogenic Bacteria. Bangladesh Journal of Mushroom, June 2009; 3(1): 9-13. |
CONFERENCE PAPER
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BOOK CHAPTER
2021 |
Shawan, MMAK; Islam, N; Aziz, S; Khatun, N; Sarker, SR; Hossain, M; Hossan, T; Morshed, M; Sarkar, M; Shakil, MS; Rahman, MN; Begum, MHA and Hasan, MA. Fabrication of Xanthan gum:Gelatin (Xnt:Gel) Hybrid Composite Hydrogels for Evaluating Skin Wound Healing Efficacy. Current Advances in Chemistry and Biochemistry Vol. 9, B P International, 8 July 2021, Page: 130-143. |
AWARD
· Awarded by “Jahangirnagar University Merit Scholarship” in the year 2011 based on M.Sc. result.
· Awarded by “Jahangirnagar University Merit Scholarship” in the years 2007, 2008, 2009, and 2010 based on the results of each year during the entire period of the B.Sc. program.
THESIS SUPERVISION
2021 |
1. Designing an effective peptide vaccine against the S-protein of SARS-CoV-2 by Sajal Kumar Halder (M.Sc level). |
2017 |
2. In silico subtractive genomics identify and characterize MG_476 (hypothetical protein of Mycoplasma genitalium G37) as a potential drug target and vaccine candidate by Naznin Jahan (M.Sc level). |
2016 |
3. Study on the preparation and characterization of gelatin-xanthan gum-based biodegradable hydrogel and evaluation of their potency in rat skin wound animal model by Nazmul Islam (M.Sc level). 4. Identification and characterization of a novel drug & vaccine target HPAG1_0576 from the hypothetical proteins of Helicobacter Pylori HPAG1; an in silico approach by Hanan Ashrafi (M.Sc level). |
WORKSHOP
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OTHER
PEER REVIEWER
Reviewing Year |
Journal Name |
2023 |
2 articles for the journal “Archives of Microbiology” published by Springer Nature. |
2022 |
1 article for the journal “Indian Journal of Experimental Biology” published by the National Institute of Science Communication and Information Resources. |
RESEARCH GRANTS RECEIVED AS PROJECT INVESTIGATOR
2014-2023 |
Achieved Jahangirnagar University and University Grants Commission (UGC) funded research grants for the fiscal year of 2014-2015, 2015-2016, 2016-2017, 2017-2018, 2018-2019, 2019-2020, 2020-2021, 2021-2022, and 2022-2023. |
2022-2023 |
Achieved a special research allocation project (SRG-225604) as a co-investigator funded by the Ministry of Science and Technology, Bangladesh. Achieved a research allocation project as a co-investigator funded by the University Grants Commission (UGC) of Bangladesh. |
AFFILIATIONS
Bangladesh |
Graduate Biochemists Association (GBA), Bangladesh; Bangladesh Society for Biochemistry and Molecular Biology (BSBMB); University Teachers Association of Bangladesh (UTAB); Jahangirnagar University Teachers Association (JUTA), Bangladesh; Jahangirnagar University Alumni Association (JUAA), Bangladesh; and Notre Dame College Alumni Association (NDCAA), Bangladesh. |
USA |
American Society for Microbiology (ASM Membership Number-56897648). |
SKILLS AND TECHNIQUES
Laboratory Skills |
Skills in Microbiology: Bacterial cell culture, isolation & characterization of bacterial strains, in vitro antibiotic susceptibility testing & MIC determination, biofilm assay, cell counting by flow cytometer, light, fluorescent and confocal microscopy.
Skills in Biochemistry and Molecular Biology: Fluorescence spectroscopy, determination of various biochemical/clinical parameters from blood samples, isolation of DNA & RNA, conventional and Real-Time PCR, plasmid profiling, ribotyping, LPO assay, synthesis & characterization of nanoparticles, hemocompatibility & biocompatibility assay, histopathological examination, Restriction Fragment Length Polymorphism (RFLP), and gel electrophoresis.
Skills in Immunology: ELISA, immunochromatographic test, and immunofixation electrophoresis.
Skills in Animal Handling: Rat and mice.
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Bioinformatics Skills |
In silico vaccine design, identification of potential drug targets, in silico promoter detection, in silico RNAi (siRNA) detection, drug design, protein networking, homology modeling, molecular docking, ADMET analysis, in silico molecular target prediction, in silico biological activity prediction, in silico true tRNA detection, molecular dynamics simulation, QM/MM for protein, characterization of drugs, organic & inorganic compounds, and optimization by DFT, HF and MP2 theories. |
Teaching
Course Code | Course Title | Semester/Year |
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BMB 1152 | Biophysical Chemistry | 1st Year 1st Semester |
BMB 2103 | Human Physiology II | 2nd Year 1st Semester |
BMB 302 | Metabolism III | Third |
BMB 409 | Tumor Biology | Fourth |
Academic Info
Period: Academic Session- 2010-2011 (Exam of 2011, Exam held on 2012 and Result published on 2012)
Master of Science (M.Sc.) in Biochemistry and Molecular Biology (Thesis Group), Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Thesis Title: Regional variation in antibiotic resistance pattern and molecular analysis among clinical isolates of Vibrio cholerae O1 isolated during cholera outbreak in 2009-2010.
Grade: GPA - 3.75 Out of 4.0 (75% marks); Position 1st out of 34 students.
Courses Studied: Biochemistry of Drugs and Diseases, Biochemical, Molecular and Immunodiagnostics, Emerging Frontiers of Molecular Biology, Food and Industrial Biotechnology, Thesis, Seminar and Viva-Voce.
Thesis Conducted at Environmental Microbiology Laboratory (EML), Centre for Food and Waterborne Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.
Period: Academic Session- 2005-2006 (Exam of-2010, Exam held on 2011 and Result published on 2011)
Bachelor of Science (B.Sc.) in Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Grade: cGPA - 3.78 Out of 4.0 (75.6% marks), Position 4th out of 36 students.
Courses studied:
Part-I: Basic Biochemistry, Basic Molecular Biology, Bio-Organic Chemistry, Bio-Physical Chemistry, Botany, Zoology, Practical Biochemistry-I, English, Practical Botany and Zoology and Viva-Voce.
Part-II: Enzymes and Bioenergetics, Reaction Mechanism, Metabolism-I, Human Physiology, Basic Microbiology, Molecular Genetics, Introductory Immunology, Biostatistics, Practical Biochemistry-II, Biostatistics and Computer Practical and Viva-Voce.
Part-III: Metabolism-II, Introductory Pharmaceutical Science, Molecular Biology-I, Endocrinology, Clinical Biochemistry, Immunology, Plant Biochemistry, Genetic Engineering, Practical Biochemistry-III and Viva-Voce.
Part-IV: Human Nutrition, Neurochemistry, Advanced Molecular Biology, Genomics and Bioinformatics, Natural Products and Antibiotics, Cell Physiology, Virology and Cancer Biology, Practical Biochemistry-IV and Viva-Voce.
Period: 2003-2004 (Exam held on 2005 and Result published on 2005)
Higher Secondary School Certificate Examination (HSC) (Science Group), Notre Dame College, Motijheel, Dhaka-1000, Bangladesh.
Grade: GPA - 4.80 Out of 5.0.
Period: 1993-2003 (Exam held on 2003 and Result published on 2003)
Secondary School Certificate Examination (SSC) (Science Group), Government Science College Attached High School, Tejgaon, Dhaka-1215, Bangladesh.
Grade: GPA - 4.88 Out of 5.0 (Without 4th Subject).
Experience
Position: Assistant Professor
Period: November 30, 2017- Till date
Faculty Member (Assistant Professor), Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Responsibilities:
Lecture preparation, class conduction (both practical & theory), student counseling, member of various examination committees (question setting, conducting final exam and assessing answer papers), in charge of seminar library, supervision of MS thesis students, applying for research grants and conducting research.
Position: Lecturer
Period: October 26, 2014- November 29, 2017
Faculty Member (Lecturer), Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Responsibilities:
Lecture preparation, class conduction (both practical & theory), student counseling, member of various examination committees (question setting, conducting final exam and assessing answer papers), supervision of MS thesis students and conducting research.
Position: Research Officer
Period: May 1, 2014- October 24, 2014
Research Officer at Environmental Microbiology Laboratory, Centre for Food and Waterborne Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.
Job Responsibilities:
- Water samples collection from household as well as aquatic environment from different parts of Bangladesh.
- Determination of microbial load/contamination in water samples.
- Microscopic analysis of water samples for Zooplankton and Phytoplankton.
- Measurement of different physico chemical parameters of water sample i.e. temperature, pH, TDS, total hardness, salinity, turbidity etc.
- Microbial culture, Serology, Biochemical test, Antibiotic susceptibility testing and Molecular techniques eg. DNA extraction, Plasmid profiling, Polymerase Chain Reaction, Ribotyping (use fluorescinated probes), gel electrophoresis, cell counting by flow cytometer etc.
- Isolation and Identification of V. cholerae, E. coli, Coliforms, Faecal Streptococci, Salmonella, Shigella, Pseudomonas, S. aureus and C. perfringens by conventional culture technique, serology and molecular technique.
- Detection of V. cholerae by Direct Fluorescent Antibody (DFA) and Polymerase Chain Reaction (PCR) techniques.
- Isolation, identification and characterization (cultural, biochemical, serological and molecular) of V. cholerae in patient's rectal swabs.
- Determination of the presence of toxin producing genes such as, Zot, Ace (Accessory cholera enterotoxin), ctxA etc in V. cholerae O1 strains using PCR technique.
- Detection of Salmonella, Shigella, E.coli and Pseudomonas by PCR technique using primer for species specific genes.
- Analysis of antibiotic resistance pattern among clinical and environmental isolates.
- Determination of the presence of antibiotic resistant gene in different enteric pathogens.
- Investigation of genetic diversity and clonal abundance through ribotyping by using fluoresceinated probes among V. cholerae O1 isolates.
- Maintain the rules of Biosafety & Biosecurity in laboratory and field.
- Data entry, report preparation, and result analysis.
- Review and update quality assurance manual according to ISO guidelines for laboratory test processes and results as well as train newly hired staff on ISO guidelines and bio-safety procedures.
- Maintain temperature log for sample and investigational products freeze and fridger as well as main temperature log in transit through cool box; contact with bio-medical engineering for maintenance works of medical equipments; control temperature as per guidelines.
Position: Senior Research Assistant
Period: March 2013- April 2014
Senior Research Assistant at Environmental Microbiology Laboratory, Centre for Food and Waterborne Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali, Dhaka-1212, Bangladesh.
Job Responsibilities:
- Water samples collection from household as well as aquatic environment from different parts of Bangladesh.
- Determination of microbial load/contamination in water samples.
- Microscopic analysis of water samples for Zooplankton and Phytoplankton.
- Measurement of different physico chemical parameters of water sample i.e. temperature, pH, TDS, total hardness, salinity, turbidity etc.
- Microbial culture, Serology, Biochemical test, Antibiotic susceptibility testing and Molecular techniques eg. DNA extraction, Plasmid profiling, Polymerase Chain Reaction, Ribotyping (use fluorescinated probes), gel electrophoresis, cell counting by flow cytometer etc.
- Isolation and Identification of V. cholerae, E. coli, Coliforms, Faecal Streptococci, Salmonella, Shigella, Pseudomonas, S. aureus and C. perfringens by conventional culture technique, serology and molecular technique.
- Detection of V. cholerae by Direct Fluorescent Antibody (DFA) and Polymerase Chain Reaction (PCR) techniques.
- Isolation, identification and characterization (cultural, biochemical, serological and molecular) of V. cholerae in patient's rectal swabs.
- Determination of the presence of toxin producing genes such as, Zot, Ace (Accessory cholera enterotoxin), ctxA etc in V. cholerae O1 strains using PCR technique.
- Detection of Salmonella, Shigella, E.coli and Pseudomonas by PCR technique using primer for species specific genes.
- Analysis of antibiotic resistance pattern among clinical and environmental isolates.
- Determination of the presence of antibiotic resistant gene in different enteric pathogens.
- Investigation of genetic diversity and clonal abundance through ribotyping by using fluoresceinated probes among V. cholerae O1 isolates.
- Maintain the rules of Biosafety & Biosecurity in laboratory and field.
- Data entry, report preparation, and result analysis.
- Review and update quality assurance manual according to ISO guidelines for laboratory test processes and results as well as train newly hired staff on ISO guidelines and bio-safety procedures.
- Maintain temperature log for sample and investigational products freeze and fridger as well as main temperature log in transit through cool box; contact with bio-medical engineering for maintenance works of medical equipments; control temperature as per guidelines.
Position: Elective Fellow (Thesis Student)
Period: August 2011 to November 2012
Conduct Thesis (2011-2012) entitled “Regional Variation in Antibiotic Resistance Pattern and Molecular Analysis among Clinical Isolates of Vibrio cholerae O1 Isolated During Cholera Outbreak in 2009-2010” at Environmental Microbiology Laboratory (EML), Centre for Food and Waterborne Diseases (CFWD), International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b).
Contact
Mohammad Mahfuz Ali Khan Shawan
Assistant Professor
Department of Biochemistry & Molecular Biology
Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Cell Phone: +88-01714-451833, +88-01797-547130
Work Phone: +88-02-7791045-51 Ext.: 1379
Email: mahfuz_026shawan@juniv.edu
, mahfuz_026shawan@yahoo.com